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[Involvement of Leukotrine B4 Receptors in the Inflammatory Responses and Immunological Regulation in Vitro]

[Involvement of Leukotrine B4 Receptors in the Inflammatory Responses and Immunological Regulation in Vitro]

Chun-guang Han, Huo-gao Huang, Ming Hu, Wen-yan Luo, Yue Gao, Qiong Wang, Yong-xue Liu

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009 May;25(2):273-6.

PMID: 21189570

Abstract:

Aim:
BLT1 and BLT2 were both recently cloned and identified as two subtypes of leukotrine B4 (LTB4) receptors. With the usage of U-75302 and LY255283, the specific antagonists of BLT1 and BLT2 respectively, the involvement of BLT1 and BLT2 in the inflammatory and immunological responses was in vitro explored.
Methods:
(1) To investigate inhibition of U-75302 and LY255283 on the proliferation of rat synovial cells, 3H-TdR incorporation into the cells was quantified. (2) Flow cytometric assay for interferon-gamma (IFN-gamma) and interleukine 4 (IL-4) profiles in CD4+ T lymphocytes from rat spleen was carried out to determine the ratio of Th1/Th2.
Results:
(1) For inhibition on rat synovial cells proliferation, U-75302 exerted its effect only at a high concentration of 10 micromol/L and LY255283 at the concentrations of 10 micromol/L-10 micromol/L. (2) Both U-75302 and LY255283 could elevate the percentage of Th2, but could not influence that of Th1.
Conclusion:
BLT1 and BLT2 were involved in the synovial cells proliferation change the ratio of Th1/Th2. Their meaning served as targets for prevention and treatment of infectious diseases should be emphasized.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP117690796	LY255283		117690-79-6	Price

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