Home
Resources
Publications
Involvement of Soil Bacteria in ABO Blood Mistyping

Involvement of Soil Bacteria in ABO Blood Mistyping

Naoki Takada, Chikahiro Mori, Rie Takai, Tomohiro Takayama, Yoshihisa Watanabe, Kohei Nakamura, Kazuhiro Takamizawa

Leg Med (Tokyo). 2015 Mar;17(2):128-33.

PMID: 25464834

Abstract:

The current study investigated whether ABO blood mistyping of human biological samples is induced by soil bacteria. A total of 380 bacterial strains were isolated from 50 discrete soil samples using human blood agar, and glycosidase activity evaluated for all strains using 4-nitropheny glycosides (4-nitrophenyl n-acetyl-α-D-galactosaminide, 4-nitrophenyl-α-D-galactopyranoside, 4-nitrophenyl-α-L-fucopyranoside) as substrates. Thirteen strains possessed α-galactosidase activity, and 16S rRNA sequence analysis revealed a close relatedness to the genus Bacillus. An indirect competitive enzyme-linked immunosorbent assay confirmed seven strains exhibited type B antigen degradation activity. These results demonstrated that 1.8% of the bacteria isolated from soil, were Bacillus sp., possessed galactosidase activity, and had the potential to cause ABO blood mistyping.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP23646686	4-Nitrophenyl N-acetyl-α-D-galactosaminide		23646-68-6	Price
AP7493950	4-Nitrophenyl α-D-galactopyranoside		7493-95-0	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: