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IR-783 Labeling of a Peptide Receptor for 'Turn-On' Fluorescence Based Sensing

IR-783 Labeling of a Peptide Receptor for 'Turn-On' Fluorescence Based Sensing

Eun-A Kwak, LeNaiya Kydd, Butaek Lim, Justyn Jaworski

Chemosensors (Basel). 2018 Dec;6(4):47.

PMID: 31080779

Abstract:

In this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N' terminus with the near IR fluorophore IR-783 to afford a viable fluorescent sensor after cleavage from the resin. The proof-of-concept probe utilized a biotin mimetic peptide sequence as the receptive moiety. Here we revealed a "turn-on" fluorescence enhancement upon binding of the biotin mimetic probe to its intended streptavidin target. Not all peptide-receptive moieties tested were able to generate such an enhancement upon target binding, and as such, the rationale for the observed fluorescence response properties is discussed.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP115970666	IR-783		115970-66-6	Price

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