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Is the Poly (L- Lactide- Co- Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?

Is the Poly (L- Lactide- Co- Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?

Marta Pokrywczynska, Arkadiusz Jundzill, Jan Adamowicz, Tomasz Kowalczyk, Karolina Warda, Marta Rasmus, Lukasz Buchholz, Sandra Krzyzanowska, Pawel Nakielski, Tomasz Chmielewski, Magdalena Bodnar, Andrzej Marszalek, etc.

PLoS One. 2014 Aug 27;9(8):e105295.

PMID: 25162451

Abstract:

The purpose of this study was to compare: a new five-layered poly (L-lactide-co-caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L-lactide-co-caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×10(6) cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×10(6)ADSCs; (II) SIS+ 3×10(6)ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L-lactide-co-caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS74356	Poly(L-lactide-co-caprolactone)			Price

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