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Isolation and Characterization of an Oxygen Radical Absorbance Activity Peptide From Defatted Peanut Meal Hydrolysate and Its Antioxidant Properties

Isolation and Characterization of an Oxygen Radical Absorbance Activity Peptide From Defatted Peanut Meal Hydrolysate and Its Antioxidant Properties

Lin Zheng, Guowan Su, Jiaoyan Ren, Longjian Gu, Lijun You, Mouming Zhao

J Agric Food Chem. 2012 May 30;60(21):5431-7.

PMID: 22577732

Abstract:

Defatted peanut meal hydrolysate (DPMH) was purified using ultrafiltration, gel filtration chromatography, and high-performance liquid chromatography. A tripeptide with strong oxygen radical absorbance capacity (ORAC) was isolated and identified as Tyr-Gly-Ser by ESI-MS/MS. It was then synthesized to measure its antioxidant properties in different systems. The ORAC value of Tyr-Gly-Ser was 3-fold higher than that of glutathione (GSH), and it displayed a stronger protective effect on linoleic acid peroxidation and H(2)O(2)-induced oxidative injury in rat pheochromocytoma line PC12 cells than GSH (p < 0.05). However, Tyr-Gly-Ser showed negligible DPPH radical scavenging activity, reducing power, and no metal chelating ability. The results suggested that Tyr-Gly-Ser displayed antioxidant activity via the hydrogen atom transfer mechanism, and the Tyr at the N-terminal was the hydrogen donor. The ORAC assay was recommended as a reliable and effective method to measure the antioxidant activity in the course of antioxidant peptide isolation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AS25103	Defatted peanut meal			Price

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