Isolation and Characterization of Highly Purified Streptavidin Obtained in a Two-Step Purification Procedure From Streptomyces Avidinii Grown in a Synthetic Medium

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Isolation and Characterization of Highly Purified Streptavidin Obtained in a Two-Step Purification Procedure From Streptomyces Avidinii Grown in a Synthetic Medium

Isolation and Characterization of Highly Purified Streptavidin Obtained in a Two-Step Purification Procedure From Streptomyces Avidinii Grown in a Synthetic Medium

M Suter, J Cazin Jr, J E Butler, D M Mock

J Immunol Methods. 1988 Oct 4;113(1):83-91.

PMID: 3049826

Abstract:

A method is described for isolation of streptavidin from cultures of Streptomyces avidinii grown in a synthetic culture medium for 6-10 days. Streptavidin is precipitated directly from culture supernatant fluid using 80% ammonium sulfate, and the precipitate is dialyzed against water and centrifuged at 40,000 X g for 60 min. The absorbency coefficient at 280 nm of purified streptavidin was estimated to be 31.7142 +/- 0.1806 for a 1% solution. The protein appeared to be greater than 90% homogeneous by gel permeation chromatography and polyacrylamide gel electrophoresis. No biotin-binding molecules less than 70 kDa in size were detected at any step during the purification of streptavidin. Streptavidin was able to maintain a stable crosslink between two biotinylated molecules in a solid-phase assay. Streptavidin purified by this method was stable in 50% glycerol/water at -20 degrees C for more than 1 year. Lyophilization or iodination did not produce apparent damage to the protein.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9013201	Streptavidin from Streptomyces avidinii		9013-20-1	Price

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