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Isolation and Characterization of Two N-acetyl-D-lactosamine Specific Lectins From Tubers of Arisaema Intermedium Blume and A. Wallichianum Hook F

Isolation and Characterization of Two N-acetyl-D-lactosamine Specific Lectins From Tubers of Arisaema Intermedium Blume and A. Wallichianum Hook F

Manpreet Kaur, Jatinder Singh, Sukhdev Singh Kamboj, Jagmohan Singh, Amandeep Kaur, S K Sood, A K Saxena

Indian J Biochem Biophys. 2005 Feb;42(1):34-40.

PMID: 23923579

Abstract:

Two new lectins were purified from the tubers of Arisaema intermedium Blume and A. wallichianum Hook. f. (family: Araceae) by affinity chromatography on asialofetuin-linked amino activated silica beads. The bound lectins were eluted with 0.1 M glycine-HCl, pH 2.5. They gave a single band corresponding to subunit M(r) 13.4 kDa in SDS-PAGE, pH 8.3. On gel filtration chromatography, the lectins showed a M(r) of 51.2 kDa, suggesting a homotetrameric structure. Both the lectins gave a single peak on size exclusion HPLC and cation-exchange columns and a single band on PAGE, pH 4.5. However, like other monocot lectins, they gave multiple bands in isoelectric focusing and at PAGE 8.3. The lectins were inhibited by N-acetyl-D-lactosamine (LacNAc), a disaccharide and asialofetuin, a complex desialylated serum glycoprotein. They had no requirement for divalent metal ions i.e., Ca2+ and Mn2+ for their activity and were found to be mitogenic towards human lymphocytes. A. intermedium showed antiproliferative effect against various human cancer cell lines in vitro.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP32181592	N-Acetyl-D-lactosamine		32181-59-2	Price

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