Home
Resources
Publications
Isolation and Identification of the Native Population Bacteria for Bioremediation of High Levels of Arsenic From Water Resources

Isolation and Identification of the Native Population Bacteria for Bioremediation of High Levels of Arsenic From Water Resources

Mohammad Ahmadi Jebelli, Afshin Maleki, Mohammad Ali Amoozegar, Enayatollah Kalantar, Fardin Gharibi, Neda Darvish, Hamidreza Tashayoe

J Environ Manage. 2018 Apr 15;212:39-45.

PMID: 29427940

Abstract:

Health of millions of people is threatened by the risk of drinking arsenic-contaminated water worldwide. Arsenic naturally conflicts with the concept of life, but recent studies showed that some microorganisms use toxic minerals as the source of energy. Hence, the researchers should consider the development of cost-effective and highly productive procedures to remove arsenic. The current study was conducted on a native bacterial population of Seyed-Jalaleddin Spring Kurdistan, Iran. Accordingly, the arsenic amount in water samples was measured >500 μg/L by the two field and in vitro methods. Water samples were transferred to laboratory and cultured on chemically defined medium (CDM) with arsenic salts. A total of 14 native arsenic-resistant bacterial strains were isolated and after providing pure culture and performing biochemical tests, the isolates were identified using polymerase chain reaction (PCR) and 16s rRNA genomic sequencing. The potential of bacterial strains for the biotransformation of arsenic was assessed by the qualitative assessment of AgNO3 method and efficiency of arsenic speciation was determined for the first time by silver diethyldithiocarbamate (SDDC) method with an error of less than 5%. Among the isolated strains, only strain As-11 and strain As-12 showed arsenic transformation characteristics and were registered in NCBI database by the access numbers KY119262 and KY119261, respectively. Results of the current study indicated that strain As-11 had the potential of biotransformation of As(V) to As(III) and vice versa with the efficiency of 78% and 48%, respectively. On the other hand, strain As-12 had the potential for biotransformation of As(V) to As(III) and vice versa with the efficiency of 28% and 45%, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1470617	Silver diethyldithiocarbamate		1470-61-7	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: