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[Isolation and Purification of Human Brain Creatine Kinase (CK-BB)]

Z Lu

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1991 Aug;13(4):298-301.

PMID: 1839527

Abstract:

A improved method for isolation and purification of CK-BB from human brain is introduced. CK-BB of fraction IV was purified to approximately 306.9 fold as compared with homogenate of human brain. The specific activity was 322.2 IU/mg protein. The recovery of creatine kinase activity was 52.8%. This fraction was analyzed by SDS-PAGE. It showed a single major band with an estimated molecular weight of 43000 when it stained with coomassie blue. Purity of fraction V was much better than fraction IV, but some CK activity was lost.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42412031 Creatine Kinase BB Fraction human Creatine Kinase BB Fraction human Price
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