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Isolation and Purification of Phenylethanoid Glycosides From Cistanche Deserticola by High-Speed Counter-Current Chromatography

Isolation and Purification of Phenylethanoid Glycosides From Cistanche Deserticola by High-Speed Counter-Current Chromatography

Li Li, Rong Tsao, Raymond Yang, Chunming Liu, J Christopher Young, Honghui Zhu

Food Chem. 2008 May 15;108(2):702-10.

PMID: 26059151

Abstract:

Five phenylethanoid glycosides (PhGs), echinacoside, cistanoside A, acteoside, isoacteoside and 2'-acetylacteoside, were isolated and purified from Cistanche deserticola for the first time by high-speed counter-current chromatography (HSCCC) using two biphasic systems, one consisting of ethyl acetate-ethanol-water (5:0.5:4.5, v/v/v) and another of ethyl acetate-n-butanol-ethanol-water (0.5:0.5:0.1:1, v/v/v/v). A total of 28.5mg of echinacoside, 18.4mg of cistanoside A, 14.6mg of acteoside, 30.1mg of isoacteoside and 25.2mg of 2'-acetylacteoside were purified from 1412mg of the n-butanol extract of C. deserticola, each at over 92.5% purity as determined by HPLC. The structures were identified by their retention time, UV, LC-ESI-MS in the negative ion mode, and confirmed by NMR experiments. The characteristic LC-ESI-MS(n) fragmentation pattern of the five compounds is discussed, and found to be a very specific and useful tool for the structural identification of PhGs from this important medicinal plant.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP94492247	2′-Acetylacteoside		94492-24-7	Price

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