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Isolation and Purification of Schisandrol A From the Stems of Schisandra Chinensis and Cytotoxicity Against Human Hepatocarcinoma Cell Lines

Isolation and Purification of Schisandrol A From the Stems of Schisandra Chinensis and Cytotoxicity Against Human Hepatocarcinoma Cell Lines

Lijie Zhu, Bin Li, Xiuying Liu, Guohui Huang, Xianjun Meng

Pharmacogn Mag. Jan-Mar 2015;11(41):131-5.

PMID: 25709222

Abstract:

Background:
Schisandrol A, a lignan with anticancer effects, is one of the representative components that identifies Schisandra chinensis.
Objective:
A method for purifying schisandrol A from the stems of S. chinensis was established using an octadecylsilyl (ODS) column combined with preparative high-performance liquid chromatography (HPLC).
Materials and methods:
Crude extracts obtained from the stems of S. chinensis using 70% ethanol were separated on an AB-8 macroporous resin column and then eluted with a graded ethanol series. After 70% methanol was used in an ODS column separation, preparative HPLC was used for subsequent purification. The structure was identified by electrospray ionization-mass spectrometry, (1)H-nuclear magnetic resonance, and (13)C-nuclear magnetic resonance. HepG2 and Bel-7402 hepatocellular carcinoma cell lines were used for toxicological evaluation.
Results:
21.4 mg of schisandrol A with a purity of 95.2% were collected. The cytotoxicity of the ODS-purified sample and schisandrol A were significantly stronger than that of a resin-purified sample.
Conclusion:
Schisandrol A was successfully extracted from the stems of S. chinensis and separated with an ODS column combined with preparative HPLC. The samples obtained during the purification process showed different levels of cytotoxicity on the HepG2 and Bel-7402 hepatocellular carcinoma cell lines.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7432282-A	Schisandrol A		7432-28-2	Price

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