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Isolation of a Novel N-acetylglucosamine-specific Lectin From Fresh Sclerotia of the Edible Mushroom Pleurotus Tuber-Regium

Hexiang Wang, T B Ng

Protein Expr Purif. 2003 Jun;29(2):156-60.

PMID: 12767804

Abstract:

An N-acetylglucosamine-binding lectin with a molecular mass of 32kDa was isolated from fresh sclerotia of the edible mushroom Pleurotus tuber-regium. Its N-terminal sequence exhibited some similarity to that of Agaricus bisporus lectin. The isolation procedure was simple, involving (NH(4))(2)SO(4) precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on N-acetyl-D-glucosamine-agarose, and gel filtration by fast protein liquid chromatography on Superdex 75. The lectin exhibited hemagglutinating activity toward trypsinized rabbit erythrocytes but not toward untrypsinized rabbit erythrocytes.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42410963 N-Acetyl-D-glucosamine-Agarose N-Acetyl-D-glucosamine-Agarose Price
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