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Isolation of Antigen-Specific, Antibody-Secreting Cells Using a Chip-Based Immunospot Array

Isolation of Antigen-Specific, Antibody-Secreting Cells Using a Chip-Based Immunospot Array

Hiroyuki Kishi, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, Atsushi Muraguchi

Methods Mol Biol. 2019;1904:147-162.

PMID: 30539469

Abstract:

Antigen-specific monoclonal antibodies are useful tools to detect very small amounts of antigenic materials and are applicable for antibody therapeutics. To produce mouse monoclonal antibodies, a hybridoma between B lymphocytes and myeloma cells is used to produce antigen-specific monoclonal antibodies. However, a good hybridoma system is not available to obtain human monoclonal antibodies. To produce antigen-specific human monoclonal antibodies, transformation of B lymphocytes with Epstein-Barr viruses or a phage-display system is used. Here, we describe the screening of antigen-specific, antibody-secreting cells using microwell array chips to obtain antigen-specific human monoclonal antibodies. The system can be applied to screen antigen-specific, antibody-secreting cells from any animal species.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248950	Anti-STUB1/CHIP antibody, mouse monoclonal			Price

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