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Isolation of β-carotene, α-carotene and Lutein From Carrots by Countercurrent Chromatography With the Solvent System Modifier Benzotrifluoride

Isolation of β-carotene, α-carotene and Lutein From Carrots by Countercurrent Chromatography With the Solvent System Modifier Benzotrifluoride

Michael Englert, Simon Hammann, Walter Vetter

J Chromatogr A. 2015 Apr 3;1388:119-25.

PMID: 25728658

Abstract:

A carotenoid purification method with dual-mode countercurrent chromatography (CCC) for β-carotene, α-carotene and lutein from a fresh carrot extract was developed. The fluorinated liquid benzotrifluoride (IUPAC name: (trifluoromethyl)benzene) was used as a novel modifier in the non-aqueous ternary solvent system n-hexane/benzotrifluoride/acetonitrile. The ternary phase diagram of the type I solvent system was used to select two-phase solvent mixtures which enabled an efficient preparative separation of α-carotene, β-carotene and lutein from concomitant pigments in crude carrot extract. By means of the modifier, high separation factors (α ≥ 1.2) were obtained, allowing baseline resolution between α-carotene and β-carotene due to specific chemical interactions such as π-π molecular interactions. After optimizing the injection step with a pseudo-ternary phase diagram, 51 mg of β-carotene, 32 mg of α-carotene and 4 mg of lutein could be isolated from 100.2mg crude carrot extract in a short time and with high purities of 95% and 99% by using dual-mode CCC, respectively. Temperatures > 22°C had a negative impact on the separation of α-carotene and β-carotene.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7488995	α-Carotene		7488-99-5	Price

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