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Isolation of Flavonoids From Delonix Elata and Determination of Its Rutin Content Using Capillary Electrophoresis

Isolation of Flavonoids From Delonix Elata and Determination of Its Rutin Content Using Capillary Electrophoresis

Areej Mohammed Al-Taweel, Maged Saad Abdel-Kader, Ghada Ahmed Fawzy, Shagufta Perveen, Hadir Mohamed Maher, Nourah Zoman Al-Zoman, Mona Mohamed Al-Shehri, Haya Al-Johar, Hessa Al-Showiman

Pak J Pharm Sci. 2015 Sep;28(5 Suppl):1897-903.

PMID: 26525033

Abstract:

Delonix elata (L.) Gamble (Fabaceae) is an important, traditionally used plant in Saudi Arabia. It is used to relieve rheumatic pain, flatulence and the seeds are employed as purgatives. The aim of the present study was to isolate chemical constituents of the n-butanol fraction (BF) of D. elata and to find out, by capillary electrophoresis (CE), percentage of rutin present in this BF. Three quercetin glycosides and one kaempferol rutinoside were isolated from the BF of aerial parts of D. elata; namely, Quercetin 3-O-rutinoside-7-O-glucoside (1), Quercetin 3,7-diglucoside (2), Quercetin 3-O-rutinoside (RUT) (3) and Kaempferol 3-O-rutinoside (4). Rutin, an active constituent has been reported to possess good pharmacological as well as therapeutic potentials. A sensitive and rapid procedure for quantitative determination of RUT by capillary electrophoresis was developed and its content was found to be 7.349 mg/gm, relative to n-butanol fraction and 18.373 mg%, relative to the dry powder of D. elata. The method could be recommended for approval and use in the pharmaceutical and food industries.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP30311616	Quercetin 3-O-rutinoside-7-O-glucoside		30311-61-6	Price

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