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Isolation of Isomangiferin From Honeybush (Cyclopia Subternata) Using High-Speed Counter-Current Chromatography and High-Performance Liquid Chromatography

Isolation of Isomangiferin From Honeybush (Cyclopia Subternata) Using High-Speed Counter-Current Chromatography and High-Performance Liquid Chromatography

Dalene de Beer, Gerold Jerz, Elizabeth Joubert, Victor Wray, Peter Winterhalter

J Chromatogr A. 2009 May 8;1216(19):4282-9.

PMID: 19272608

Abstract:

Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP24699169	Isomangiferin		24699-16-9	Price

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