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Isolation of Three Novel Cholinergic Neuron-Specific Gangliosides From Bovine Brain and Their in Vitro Syntheses

Isolation of Three Novel Cholinergic Neuron-Specific Gangliosides From Bovine Brain and Their in Vitro Syntheses

F Irie, S Kurono, Y T Li, Y Seyama, Y Hirabayashi

Glycoconj J. 1996 Apr;13(2):177-86.

PMID: 8737242

Abstract:

In the present study, three extremely minor but novel Chol-1 antigens, termed X1, X2, and X3 have been isolated from bovine brain gangliosides. Based on the results of sialidase degradation, TLC-immunostaining with anti-Chol-1 antibody and fast atom bombardment mass spectrometry, their chemical structures were identified as: III6NeuAc-GgOse4Cer (X1: GM1 alpha) III6NeuAc,II3NeuAc-GgOse4Cer (X2: GD1a alpha) III6NeuAc,II3NeuAc-NeuGc-GgOse4Cer (X3: GT1b alpha) The yields of GM1 alpha, GD1a alpha, and GT1b alpha, were approximately 150, 20, and 10 micrograms, respectively, from 10 g of the bovine brain ganglioside mixture. In conjunction with our previous observations, all gangliosides with anti-Chol-1 reactivity were found to contain a common sialyl alpha 2-6 N-acetylgalactosamine residue, indicating that this unique sialyl linkage is the specific antigenic determinant. We subsequently examined the biosynthesis of the three novel Chol-1 gangliosides using rat liver Golgi fraction as an enzyme source. The results showed that GM1 alpha, GD1a alpha, and GT1b alpha were synthesized from asialo-GM1, GM1a, and GD1b, respectively, by the action of a GalNAc alpha 2-6sialyltransferase.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP71012196-A	Asialoganglioside GM1 from bovine brain		71012-19-6	Price

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