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[Isolation of Yeast Protoplasts Using Various Preparations of the Hepato-Pancreatic Juice of Helix Pomatia]

M Diatewa, I Viard, A J Stahl

Biochimie. 1981 Jan;63(1):67-9.

PMID: 7011422

Abstract:

Conversion of large amounts of Saccharomyces cerevisiae cells to protoplasts is studied using various preparations extracted from Helix pomatia hepato-pancreatic juice. The most favourable yield in two hours incubations (88 per cent) is obtained with 20 ml cytohelicase, a chitinase and glucanase enriched extract, per 400 g of yeast cells, harvested at the end of the logarithmic growth phase and preincubated in presence of 2-mercaptoethanol.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP42613296 Cytohelicase from Helix pomatia Cytohelicase from Helix pomatia 42613-29-6 Price
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