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J Blood Group Active Lipoproteins Extracted From Bovine Erythrocytes

J Blood Group Active Lipoproteins Extracted From Bovine Erythrocytes

B Kirchhof, O W Thiele, M E Petri, J Koch

Anim Blood Groups Biochem Genet. 1976;7(1):51-8.

PMID: 970674

Abstract:

Ghosts of J-positive bovine erythrocytes were frozen overnight, thawed the next day, washed with ion-free water, and then extracted with a water-n-pentanol mixture. After centrifugation, the aqueous phase contained a great deal of the membrane proteins and lipids in a soluble form along with the J blood-group activity. After preparative ultracentrifugation, about 2/3 of the J activity were recovered in the high-density lipoprotein fraction, while the low-density lipoprotein fraction contained 1/3 J activity. This result is consistent with out finding that on treatment of the stroma lipoproteins with dextran sulphate, about 2/3 of J activity were recovered in the supernatant, 1/3 in the precipitate. These fractions obtained by dextran sulphate treatment were characterized by protein and lipid assay.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42415091	Stroma from bovine erythrocytes			Price

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