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Label-free Detection Microarray for Novel Peptide Ligands Screening Base on MS-SPRi Combination

Label-free Detection Microarray for Novel Peptide Ligands Screening Base on MS-SPRi Combination

Weizhi Wang, Di Zhang, Zewen Wei, Zihua Wang, Xiangli Bu, Shu Yang, Qiaojun Fang, Zhiyuan Hu

Talanta. 2015 Mar;134:705-711.

PMID: 25618725

Abstract:

Peptides ligands with high affinity and high specificity towards specific targets is catching a good deal of interests in biomedical field. Traditional peptide screening procedure involves selection, sequencing and characterization and each step is time-consuming and labor-intensive. The combination between different analytical methods could provide an integrated plan for efficient peptide screening. We report herein a label-free detection microarray system to facilitate the whole one-bead-one-compound (OBOC) peptide screening process. A microwell array chip with two identical units can trap the candidate peptide beads in one-well-one-bead manner. Peptides on beads were photo-released in situ in the well and partly transferred to two identical chips for Surface Plasmon Resonance imaging (SPRi), and peptide left in the bi-unit microwell array chip was remain for in situ single bead sequencing by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Using the bi-unit imprinted chip system, affinity peptides towards AD protein were efficiently screened out both qualitatively and quantitatively from 10(4) candidates. The method provides a universal solution for high efficiency and high throughput ligands screening.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP168425647	Compound 401 - CAS 168425-64-7		168425-64-7	Price

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