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Labeling a Protein With Fluorophores Using NHS Ester Derivitization

Jagpreet S Nanda, Jon R Lorsch

Methods Enzymol. 2014;536:87-94.

PMID: 24423269

Abstract:

N-hydroxysuccinimde (NHS) ester-mediated derivitization involves the reaction of this amine-reactive group with the primary amines of a protein or a biomolecule. Using NHS chemistry allows one to conjugate various fluorescent probes, biotin, and cross-linkers to primary amines. For example, we use NHS ester chemistry to fluorescently label the amino terminus of a protein with the dye, 5-(and-6)-carboxyfluorescein, succinimidyl ester (5(6)-FAM, SE).

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42418258 Dy-615 NHS ester Dy-615 NHS ester Price
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