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Lactate-sodium Cotransport in Rat Renal Brush Border Membranes

Lactate-sodium Cotransport in Rat Renal Brush Border Membranes

M Barac-Nieto, H Murer, R Kinne

Am J Physiol. 1980 Nov;239(5):F496-506.

PMID: 6159793

Abstract:

Brush border membrane vesicles were obtained from rat kidney cortex through a calcium precipitation method and their transport properties for lactate were studied by a rapid-filtration technique. Transient concentrative uptake of L-lactate was observed in the presence of inwardly directed NaCl gradient, but not in the presence of a KCl, LiCl, RbCl, CsCl, or choline chloride gradient. The sodium-dependent L-lactate uptake was saturable and was inhibited by D-lactate. The activation curve with sodium was hyperbolic. Maneuvers that render the inside of the vesicle more negative stimulated sodium-dependent L-lactate uptake, suggesting an electrogenic transfer of L-lactate and sodium. An L-lactate gradient also accelerates the sodium movement across the brush border membrane. Studies on the pH dependency of L-lactate transport and on the effect of L-lactate on proton movements across the brush border membrane indicate that there is little contribution of nonionic diffusion and/or of lactate-H+ cotransport to the transfer of L-lactate across the renal brush border membrane. In summary, sodium-lactate cotransport is the major mechanism for L-lactate transfer across the renal brush border membrane.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP888724514	2-Hydroxyethyl-trimethylammonium L-(+)-lactate		888724-51-4	Price

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