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Lafutidine Inhibits Helicobacter Pylori-Induced interleukin-8 Production in Human Gastric Epithelial Cells

Lafutidine Inhibits Helicobacter Pylori-Induced interleukin-8 Production in Human Gastric Epithelial Cells

Yoshihisa Nozawa, Katsushi Nishihara, Yushiro Akizawa, Naoki Orimoto, Motoko Nakano, Tatsuya Uji, Hirofusa Ajioka, Atsuhiro Kanda, Naosuke Matsuura, Mamoru Kiniwa

J Gastroenterol Hepatol. 2004 May;19(5):506-11.

PMID: 15086593

Abstract:

Background and aim:
Attachment of Helicobacter pylori to gastric epithelial cells leads to the production of chemokines, such as interleukin-8 (IL-8), which in turn activate and recruit neutrophils to the site of infection. Lafutidine [(+/-)-2-(furfurylsulfinyl)-N-(4-(4-(piperidinomethyl)-2-pyridyl)oxy-(Z)-2-butenyl)acetamide] is a new type of antiulcer drug that possesses an antisecretory action as well as gastroprotective activity, independent of its antisecretory action. In the present study, we examined the effects of lafutidine on H. pylori-induced IL-8 release and H. pylori adhesion to MKN45 cells.
Methods:
MKN45 cells were stimulated with H. pylori, tumor necrosis factor (TNF)-alpha, or IL-1beta, then IL-6 and IL-8 levels in the culture supernatants were determined with a specific enzyme-linked immunosorbent assay kit.
Results:
Lafutidine significantly inhibited both the release of IL-8 induced by H. pylori and the adhesion of H. pylori to cells in a dose-dependent manner. These properties of lafutidine are unrelated to the blockade of histamine H(2)-receptors, because the same effects have not been observed with other H(2)-receptor antagonists, such as cimetidine and famotidine. Lafutidine also significantly inhibited H. pylori-induced IL-6 release. Both TNF-alpha and IL-1beta-induced IL-8 releases, conversely, were little affected by lafutidine up to a concentration of 10(-5) M.
Conclusions:
These results suggest that lafutidine inhibits IL-8 release by inhibiting H. pylori adherence to gastric epithelial cells, indicating a novel mechanism by which lafutidine protects against the mucosal inflammation associated with H. pylori infection.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP118288087	Lafutidine		118288-08-7	Price

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