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Latent Redox Reporter of 4-Methoxyphenol as Electrochemical Signal Proxy for Real-Time Profiling of Endogenous H 2 O 2 in Living Cells

Latent Redox Reporter of 4-Methoxyphenol as Electrochemical Signal Proxy for Real-Time Profiling of Endogenous H 2 O 2 in Living Cells

Kesavan Manibalan, Shuang Han, Yajing Zheng, Haifang Li, Jin-Ming Lin

ACS Sens. 2019 Sep 27;4(9):2450-2457.

PMID: 31448596

Abstract:

Hydrogen peroxide (H2O2) plays a persuasive role in the human cell physiology. Developing an efficient assay platform and a highly sensitive tracking and quantification of H2O2 in a physiological system is crucial to understand the neoplastic changes and/or redox homeostasis of cells. In this study, a novel turn-on latent electrochemical redox probe coupled with electrocatalytic signal amplification strategy is proposed. A custom-made readily available turn-on latent electrochemical probe 4-methoxyphenylboronic acid pinacol ester (4-MPBP) have been designed for the selective detection of endogenous H2O2 in live cells. The electrochemical probe composed of a latent electrochemical reporter (4-methoxy phenol, 4-MP) bearing a recognition unit (boronic acid pinacol ester) for H2O2 sensing. The selective analyte-triggered chemical transformation releases free electrochemical reporter 4-MP. The amount of H2O2 was evaluated electrochemically at glassy carbon electrode (GCE) with a broad detection range of 0.5 μM-1 mM. An amplified signal response of released 4-MP to build a highly sensitive assay tool has been achieved via replacing the GCE transducer electrode with polydopamine@carbonnanotube-molebtinumdisulfie hybrid modified GCE as it delivered an exceptional dynamic detection range of 0.01-100 μM. The innovative blend of electrochemical molecular probe strategy, with electrocatalytic signal amplification technique has delivered outstanding assay performance at trace level sensing of H2O2. Next, we set up a platform for real-time in vivo monitoring of the endogenously produced H2O2 in Caco-2 and MCF-7 cells through spermine-polyamine analogue and phorbol 12-myristate 13-acetate induction in SSAT/PAO gene and protein kinase C, respectively. As expected, the 4-MPBP latent probe coupled with electrocatalytic signal amplification strategy delivered outstanding performance for in situ H2O2 release and tracking over time.

Chemicals Related in the Paper:

Catalog Number	Product Name	CAS Number	Price
AP1050423874	4-Carboxy-2-fluorophenylboronic acid pinacol ester	1050423-87-4	Price
AP569343095	9,9-Dimethylfluorene-2-boronic acid pinacol ester	569343-09-5	Price
AP864754198	1-(Thiophen-2-ylmethyl)-1H-pyrazole-4-boronic acid pinacol ester	864754-19-8	Price
AP936250178	2,4-Dimethoxypyrimidine-5-boronic acid pinacol ester	936250-17-8	Price
LS793301	4-methoxyphenylboronic acid, pinacol ester		Price
LS793429	2-Morpholinothiazole-4-boronic acid pinacol ester		Price

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