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[LC/MS/MS Analysis of the Metabolites of Lathyrane Diterpenoids in Caco-2 Cells]

[LC/MS/MS Analysis of the Metabolites of Lathyrane Diterpenoids in Caco-2 Cells]

Si-li Tang, Ling-ling Zhang, Wen-jing Huang, Hubiao Chen, Jian-ye Zhang

Zhong Yao Cai. 2016 Aug;39(8):1771-4.

PMID: 30204381

Abstract:

Objective:
To identify the metabolites of euphorbetin L1,euphorbetin L2,euphorbetin L8 and 6( 17),12( E)-lathyrol-5,15-diacetate-3-phenylacetate in Caco-2 cells by LC/MS/MS.
Methods:
Caco-2 cells were cultured with 100 μg/mol lathyrane diterpenoid for 3,6,12 h,respectively. Then the samples were collected,purified and identified by LC/MS/MS.
Results:
The major metabolites of euphorbetin L1 were two methylated products which were obtained after hydrolysis of the ester. The major metabolites of euphorbetin L2,euphorbetin L8 and 6( 17),12( E)-lathyrol-5,15-diacetate-3-phenylacetate were hydrolysis products of the ester.
Conclusion:
The main metabolic pathway of euphorbetin L1 is methylation and hydrolysis of the ester. The main metabolic pathway of euphorbetin L2,euphorbetin L8 and 6( 17),12( E)-lathyrol-5,15-diacetate-3-phenylacetate is hydrolysis of the ester. LC/MS/MS can identify the metabolites of euphorbetin L1,euphorbetin L2,euphorbetin L8 and 6( 17),12( E)-lathyrol-5,15-diacetate-3-phenylacetate in Caco-2 cells quickly and sensitively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP34420194	Lathyrol		34420-19-4	Price

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