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Leukotriene A4 Hydrolase in Human Leukocytes. Purification and Properties

O Rådmark, T Shimizu, H Jörnvall, B Samuelsson

J Biol Chem. 1984 Oct 25;259(20):12339-45.

PMID: 6490615

Abstract:

Leukotriene A4 hydrolase, a soluble enzyme catalyzing hydrolysis of the allylic epoxide leukotriene A4 to the dihydroxy acid leukotriene B4, was purified to apparent homogeneity from human leukocytes. The enzymatic reaction obeyed Michaelis-Menten saturation kinetics with respect to varying concentrations of leukotriene A4. An apparent KM value ranging between 20 and 30 microM was deduced from Eadie-Hofstee plots. Physical properties including molecular weight (68,000-70,000), amino acid composition, and aminoterminal sequence were determined. It was indicated that leukotriene A4 hydrolase is a monomeric protein, distinct from previously described epoxide hydrolases in liver.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP90119076 Leukotriene A4 Hydrolase human Leukotriene A4 Hydrolase human 90119-07-6 Price
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