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Levels of Matrix metalloproteinase-2 in Committed Differentiation of Bone Marrow Mesenchymal Stem Cells Induced by Kartogenin

Levels of Matrix metalloproteinase-2 in Committed Differentiation of Bone Marrow Mesenchymal Stem Cells Induced by Kartogenin

Cheng Wang, Qiaohui Liu, Xiaoyuan Ma, Guofeng Dai

J Int Med Res. 2019 Jul;47(7):3261-3270.

PMID: 31218937

Abstract:

Objective:
To measure the inductive effect of kartogenin on matrix metalloproteinase-2 levels during the differentiation of human bone marrow mesenchymal stem cells (hMSCs) into chondrocytes in vitro.
Methods:
In vitro cultured bone marrow hMSCs were grown to the logarithmic phase and then divided into three groups: control group (0 µM kartogenin), 1 µM kartogenin group and 10 µM kartogenin group. After 72 h of culture, cell proliferation and differentiation were observed microscopically. Matrix metalloproteinase-2 (MMP-2) in the cell supernatant and type II collagen levels in the cells were detected by enzyme linked immunosorbent assay and immunofluorescence staining, respectively.
Results:
Kartogenin induced the proliferation and differentiation of hMSCs. With the increase of kartogenin concentration, the level of type II collagen was increased, while the level of MMP-2 decreased.
Conclusion:
These findings indicate that kartogenin can induce hMSCs to differentiate into chondrocytes, and with the increase of kartogenin concentration, degeneration of the cartilage extracellular matrix may be inhibited.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP4727315	Kartogenin		4727-31-5	Price

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