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Light-induced Injury in Mouse Embryos Revealed by Single-Cell RNA Sequencing

Light-induced Injury in Mouse Embryos Revealed by Single-Cell RNA Sequencing

Bo Lv, Chaojie Liu, Yu Chen, Lingbin Qi, Lu Wang, Yazhong Ji, Zhigang Xue

Biol Res. 2019 Aug 29;52(1):48.

PMID: 31466525

Abstract:

Background:
Light exposure is a common stress factor in in vitro manipulation of embryos in the reproductive center. Many studies have shown the deleterious effects of high-intensity light exposure in different animal embryos. However, no transcriptomic studies have explored the light-induced injury and response in preimplantation embryos.
Results:
Here, we adopt different time-courses and illumination intensities to treat mouse embryos at the 2-cell stage and evaluate their effects on blastulation. Meanwhile, single-cell transcriptomes from the 2-cell to blastocyst stage were analyzed after high-intensity light exposure. These data show that cells at each embryonic stage can be categorized into different light conditions. Further analyses of differentially expressed genes and GO terms revealed the light-induced injury as well as the potential repair response after high-intensity lighting. Maternal-to-zygote transition is also affected by the failure to remove maternal RNAs and deactivate zygotic genome expression.
Conclusion:
Our work revealed an integrated response to high-intensity lighting, involving morphological changes, long-lasting injury effects, and intracellular damage repair mechanisms.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248890	LIGHT from mouse			Price

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