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Linear Polyethylenimine Produced by Partial Acid Hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA Delivery in Vitro

Linear Polyethylenimine Produced by Partial Acid Hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA Delivery in Vitro

Julio C Fernandes, Xingping Qiu, Françoise M Winnik, Mohamed Benderdour, Xiaoling Zhang, Kerong Dai, Qin Shi

Int J Nanomedicine. 2013;8:4091-102.

PMID: 24204139

Abstract:

Polyethylenimines (PEIs) are the most efficient synthetic vectors for gene delivery available to date. With its high charge density and strong proton-buffering effect, PEI has an ability to condense DNA and small interfering RNA at physiologic pH. However, the polymer suffers from the disadvantage of high cellular toxicity. To reduce its cellular toxicity, we synthesized linear PEIs by partial hydrolysis of poly(2-ethyl-2-oxazoline). Three linear PEIs with different hydrolysis percentages (30%, 70%, and 96%, respectively) were produced as PEI30, PEI70, and PEI96. PEI30 and PEI96 cannot be considered as suitable transfection agents because of low transfection efficiency (PEI30) or high cellular toxicity (PEI96). PEI70 displayed very weak cell toxicity. The charge density of this polymer (PEI70) was strong enough to condense DNA and small interfering RNA at a physiologic pH of 7.4. Our results also show that PEI70 was highly efficient in DNA delivery and small interfering RNA-mediated knockdown of target genes. Thus, polymers such as PEI70 appear to be very promising vectors for gene delivery.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9002986	Polyethylenimine, linear		9002-98-6	Price

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