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Liquid Chromatographic Determination of Alpha-Solasonine in Frozen Green Peas as an Indicator of the Presence of Nightshade Berries

Liquid Chromatographic Determination of Alpha-Solasonine in Frozen Green Peas as an Indicator of the Presence of Nightshade Berries

Peter Cavlovic, Mohan Mankotia, Peter Pantazopoulos, Peter M Scott

J AOAC Int. Jul-Aug 2003;86(4):759-63.

PMID: 14509436

Abstract:

Nightshade berries containing glycoalkaloids can be a contaminant in green peas. Methodology was developed to detect this contamination. The glycoalkaloid alpha-solasonine was extracted from frozen green peas with 1% (v/v) acetic acid, cleaned up on a C18 cartridge, and determined by liquid chromatography with UV detection at 200 nm. Method performance characteristics for the determination of alpha-solasonine include linearity from 140 to 1500 ng injected (r = 0.9996-0.9999); recovery ranging from 68 to 79%; limit of quantitation (LOQ) = 4.5 ppm (280 ng standard), and limit of detection = 0.64 ppm (40 ng standard). At the LOQ, the expanded uncertainty at 95% confidence was 0.38 x the reported value. The method was applied to the detection of alpha-solasonine in frozen green peas in a 2-year study of 60 samples of frozen green peas from Ontario, Canada. None of the samples contained alpha-solasonine. No unripe berries of Solanum nigrum were detected visually in the samples.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP19121585	α-Solasonine		19121-58-5	Price

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