Home
Resources
Publications
Liquid Chromatographic Determination of D- And L-amino Acids by Derivatization With O-Phthaldialdehyde and N-isobutyryl-L-cysteine. Applications With Reference to the Analysis of Peptidic Antibiotics, Toxins, Drugs and Pharmaceutically Used Amino Acids

Liquid Chromatographic Determination of D- And L-amino Acids by Derivatization With O-Phthaldialdehyde and N-isobutyryl-L-cysteine. Applications With Reference to the Analysis of Peptidic Antibiotics, Toxins, Drugs and Pharmaceutically Used Amino Acids

H Brückner, T Westhauser, H Godel

J Chromatogr A. 1995 Sep 8;711(1):201-15.

PMID: 7496491

Abstract:

In order to evaluate and extend the applicability of an analytical method that enables the quantitative and simultaneous high-performance liquid chromatographic determination of D- and L-amino acids (DL-AAs) by automated precolumn derivatization with o-phthaldialdehyde together with the chiral thiol N-isobutyryl-L-cysteine [J. Chromatogr., 666 (1994) 259] selected natural and synthetic bioactive peptides, as well as pharmaceutically used formulations of AA, were investigated and the amounts of D- and L-AA determined by fluorescence detection. Peptides containing cys(e)ine were oxidized with performic acid prior to hydrolysis with 6 M HCl, and those containing Trp were hydrolyzed with 4 M methanesulfonic acid (24 h at 110 degrees C in both cases). Peptides analyzed were the peptide antibiotics bacitracin, gramicidins A and S, polymyxin B, metanicin C, the peptide toxin malformin A and the peptide drugs D-Arg-[Hyp3,Thi5,8,D-Phe7]-bradykinin, beta-casomorphin and alpha s1-exorphin. Further, the enantiomeric ratios of pharmaceutically used AA formulations containing racemic DL-Ser, DL-Asp and DL-Met were determined, and the AA drugs L-Asp and L-Trp were tested negatively for the presence of the respective D-enantiomers. In two aqueous formulations of L-AA used for parenteral nutrition, low amounts of D-AA (0.1-0.9% with respect to certain L-AA enantiomers and of totally 128 mg and 149 mg D-AAs per liter infusion solution) were determined.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP124529028	N-Isobutyryl-L-cysteine		124529-02-8	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: