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Liquid Chromatographic Determination of Monensin in Chicken Tissues With Fluorometric Detection and Confirmation by Gas Chromatography-Mass Spectrometry

Liquid Chromatographic Determination of Monensin in Chicken Tissues With Fluorometric Detection and Confirmation by Gas Chromatography-Mass Spectrometry

K Takatsuki, S Suzuki, I Ushizawa

J Assoc Off Anal Chem. May-Jun 1986;69(3):443-8.

PMID: 3722091

Abstract:

An accurate, sensitive method is described for the determination of monensin residue in chicken tissues by liquid chromatography (LC), in which monensin is derivatized with a fluorescent labeling reagent, 9-anthryldiazomethane (ADAM), to enable fluorometric detection. Samples are extracted with methanol-water (8 + 2), the extract is partitioned between CHCl3 and water, and the CHCl3 layer is cleaned up by silica gel column chromatography. Free monensin, obtained by treatment with phosphate buffer solution (pH 3) at 0 degrees C, is derivatized with ADAM and passed through a disposable silica cartridge. Monensin-ADAM is identified and quantitated by normal phase LC using fluorometric detection. The detection limit is 1 ppb in chicken tissues. Recoveries were 77.6 +/- 1.8% at 1 ppm, 56.7 +/- 7.1% at 100 ppb, and 46.5 +/- 3.7% at 10 ppb fortification levels in chicken. Gas chromatography-mass spectrometry is capable of confirming monensin methyl ester tris trimethylsilyl ether in samples containing residues greater than 5 ppm.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP28636217	Monensin methyl ester		28636-21-7	Price

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