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Listeria Innocua Dps as a Nanoplatform for Bioluminescence Based Photodynamic Therapy Utilizing Gaussia Princeps Luciferase and Zinc Protoporphyrin IX

Listeria Innocua Dps as a Nanoplatform for Bioluminescence Based Photodynamic Therapy Utilizing Gaussia Princeps Luciferase and Zinc Protoporphyrin IX

Ali W Al-Ani, Lei Zhang, Lenny Ferreira, Lyudmila Turyanska, Tracey D Bradshaw, Neil R Thomas

Nanomedicine. 2019 Aug;20:102005.

PMID: 31048084

Abstract:

Listeria innocua DNA binding protein from starved cells (LiDps) belongs to the ferritin family and provides a promising self-assembling spherical 12-mer protein scaffold for the generation of functional nanomaterials. We report the creation of a Gaussia princeps luciferase (Gluc)-LiDps fusion protein, with chemical conjugation of Zinc (II)-protoporphyrin IX (ZnPP) to lysine residues on the fusion protein (giving Gluc-LiDps-ZnPP). The Gluc-LiDps-ZnPP conjugate is shown to generate reactive oxygen species (ROS) via Bioluminescence Resonance Energy Transfer (BRET) between the Gluc (470-490 nm) and ZnPP. In vitro, Gluc-LiDps-ZnPP is efficiently taken up by tumorigenic cells (SKBR3 and MDA-MB-231 breast cancer cells). In the presence of coelenterazine, this construct inhibits the proliferation of SKBR3 due to elevated ROS levels. Following exposure to Gluc-LiDps-ZnPP, migration of surviving SKBR3 cells is significantly suppressed. These results demonstrate the potential of the Gluc-LiDps-ZnPP conjugate as a platform for future development of an anticancer photodynamic therapy agent.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP15442645-B	Protoporphyrin IX zinc(II)		15442-64-5	Price

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