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Long-term Production of Soluble Human Fas Ligand Through Immobilization of Dictyostelium Discoideum in a Fibrous Bed Bioreactor

Long-term Production of Soluble Human Fas Ligand Through Immobilization of Dictyostelium Discoideum in a Fibrous Bed Bioreactor

Jie Chen, Heming Chen, Xiangchen Zhu, Yinghua Lu, Shang-Tian Yang, Zhinan Xu, Peilin Cen

Appl Microbiol Biotechnol. 2009 Feb;82(2):241-8.

PMID: 19015846

Abstract:

The production of recombinant glycoproteins in Dictyostelium discoideum by conventional cell culture methods was limited by low cell density as well as low growth rate. In this work, cotton towel with a good adsorption capability for D. discoideum cells was used as the immobilization matrix in an external fibrous bed bioreactor (FBB) system. With batch cultures in the FBB, the concentration of immobilized cells in the cotton fiber carrier increased to 1.37 x 10(8) cells per milliliter after 110-h cultivation, which was about tenfold higher than the maximal cell density in the conventional free-cell culture. Correspondingly, a high concentration of soluble human Fas ligand (hFasL; 173.7 microg l(-1)) was achieved with a high productivity (23 microg l(-1) h(-1)). The FBB system also maintained a high density of viable cells for hFasL production during repeated-batch cultures, achieving a productivity of 9 approximately 10 microg l(-1) h(-1) in all three batches studied during 15 days. The repeated-batch culture using immobilized cells of D. discoideum in the FBB system thus provides a good method for long-term and high-level production of hFasL.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42412944	Fas Ligand, soluble human			Price

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