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Long-term Response on Growth, Antioxidant Enzymes, and Secondary Metabolites in Salicylic Acid Pre-Treated Uncaria Tomentosa Microplants

Long-term Response on Growth, Antioxidant Enzymes, and Secondary Metabolites in Salicylic Acid Pre-Treated Uncaria Tomentosa Microplants

Silvia Sánchez-Rojo, Carlos M Cerda-García-Rojas, Fernando Esparza-García, Javier Plasencia, Héctor M Poggi-Varaldo, Teresa Ponce-Noyola, Ana C Ramos-Valdivia

Biotechnol Lett. 2015 Dec;37(12):2489-96.

PMID: 26272395

Abstract:

Objective:
To obtain micro propagated Uncaria tomentosa plantlets with enhanced secondary metabolites production, long-term responses to salicylic acid (SA) pre-treatments at 1 and 100 µM were evaluated after propagation of the plantlets in a SA-free medium.
Results:
SA pre-treatments of single node cuttings OF U. tomentosa produced long-term responses in microplants grown for 75 days in a SA-free medium. Reduction in survival rate, root formation, and stem elongation were observed only with 100 µM SA pre-treatments with respect to the control (0 + DMSO).Both pre-treatments enhanced H2O2 and inhibited superoxide dismutase and catalase activities, while guaiacol peroxidase was increased only with 1 µM SA. Also, both pre-treatments increased total monoterpenoid oxindole alkaloids by ca. 55 % (16.5 mg g(-1) DW), including isopteropodine, speciophylline, mitraphylline, isomitraphylline, rhynchopylline, and isorhynchopylline; and flavonoids by ca. 21 % (914 μg g(-1) DW), whereas phenolic compounds were increased 80 % (599 μg g(-1) DW) at 1 µM and 8.2 % (359 μg g(-1) DW) at 100 µM SA.
Conclusion:
Pre-treatment with 1 µM SA of U.tomentosa microplants preserved the survival rate and increased oxindole alkaloids, flavonoids, and phenolic compounds in correlation with H2O2 and peroxidase activity enhancements, offering biotechnological advantages over non-treated microplants.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP5171379-B	Isopteropodine		5171-37-9	Price

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