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Macrophage Differentiation Inducing Factor From Human Monocytic Cells Is Equivalent to Murine Leukemia Inhibitory Factor

Macrophage Differentiation Inducing Factor From Human Monocytic Cells Is Equivalent to Murine Leukemia Inhibitory Factor

T Abe, M Murakami, T Sato, M Kajiki, M Ohno, R Kodaira

J Biol Chem. 1989 May 25;264(15):8941-5.

PMID: 2722807

Abstract:

Human macrophage differentiation inducing factor (DIF) can induce differentiation of human myeloid leukemic cells into macrophage-like cells in vitro. A procedure is described for purification of DIF from serum-free human monocytic leukemia THP-1 cell-conditioned medium. The procedure included concentration of a conditioned medium by ultrafiltration, lentil lectin-Sepharose affinity chromatography, and fast protein liquid chromatography using Mono S and Mono Q. DIF-A of pI 9.0 and DIF-B of pI 8.8 were obtained after a final purification with a Mono Q column, and both DIF gave a single peak with a molecular weight of approximately 51,000 determined by gel chromatography. NH2-terminal amino acid analysis of DIF-A showed a noticeable homology with murine leukemia inhibitory factor. Human DIF-A was found to induce maturation of human and murine leukemic cells into both macrophage-like cells with nitro blue tetrazolium reducing activity and phagocytic cells, but was found to suppress proliferation of these leukemic cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248620	Leukemia Inhibitory Factor human			Price

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