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Making Formalin-Fixed, Paraffin Embedded Blocks

Alireza Sadeghipour, Pegah Babaheidarian

Methods Mol Biol. 2019;1897:253-268.

PMID: 30539450

Abstract:

Paraffin embedding is a standard technique used in clinical and research laboratories to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue. Formalin-fixed tissue undergoes tissue processing and then is embedded in paraffin (wax) to create a FFPE block or paraffin block. The paraffin block can be cut using a microtome to generate thin sections of tissue contained in paraffin to be stained or paraffin tissue ribbons suitable for nucleic acid extraction. In addition, the FFPE blocks can be stored at room temperature for years. Herein, we provide a basic knowledge, and introduce common methods of the paraffin embedding process.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP8002742 Paraffin Paraffin 8002-74-2 Price
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