Home
Resources
Publications
Maturation of Stretch-Induced Contractile Activity and Its Muscarinic Regulation in Isolated Whole Bladder Strips From Rat

Maturation of Stretch-Induced Contractile Activity and Its Muscarinic Regulation in Isolated Whole Bladder Strips From Rat

Thomas Gevaert, Grzegorz Owsianik, Graham Hutchings, Wouter Everaerts, Bernd Nilius, Dirk De Ridder

Neurourol Urodyn. 2010 Jun;29(5):789-96.

PMID: 18288706

Abstract:

Aim:
Besides the establishment of neural reflex pathways, developmental changes in local bladder properties probably also contribute to the onset of mature voiding reflexes. Here we explored the behavior of stretch-induced contractile activity (SIC) and its muscarinic regulation in neonatal and adult rat bladders.
Methods:
SIC was studied in bladder strips from D0, D7, D28 rat pups and adult rats (15 weeks). The responses to a non-selective [Carbachol (CA), 10(-8.5)-10(-6) M] and an M(2)-selective muscarinic agonist [arecaidine but-2-ynyl ester tosylate (ABET), 10(-9.5)-10(-7) M] were studied. The expression of M(2) and M(3) mRNA was investigated using quantitative PCR in whole bladders. The response of SIC to KCl (50 mM) and to the non-adrenergic non-cholinergic (NANC) drugs Substance P (1 microM) and alpha,beta-Methyleneadenosine 5'-triphosphate lithium salt (MATP) (1 microM) were also studied.
Results:
We found evidence for an enhanced response to the muscarinic agonists CA and ABET in neonatal bladders. This might be due to the onset of a direct contractile role for M(2), given the moderate M(2)-properties of ABET and the absence of ABET-effects on adult bladder strips. Further data showed an increased expression of both M(2) and M(3) at the mRNA level and a changed response of SIC to NANC drugs in neonatal bladder.
Conclusions:
This study reveals a changed response of SIC to muscarinic and NANC drugs in neonatal rat bladder together with changes at the muscarinic mRNA level, which might all contribute to a better insight in the role of SIC in the onset of mature voiding.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP104809203	α,β-Methyleneadenosine 5′-triphosphate lithium salt		104809-20-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: