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Measurement of Albuterol in Guinea Pig Serum by High Performance Liquid Chromatography With Fluorescence Detection

Measurement of Albuterol in Guinea Pig Serum by High Performance Liquid Chromatography With Fluorescence Detection

J R Loss 2nd, R F Orzechowski, R S Hock

Biomed Chromatogr. 2000 Feb;14(1):1-5.

PMID: 10664547

Abstract:

A sensitive, simple and reproducible high performance liquid chromatographic method for detecting and quantifying albuterol in guinea pig serum is described. A structurally related compound, bamethan, was used as an internal standard. The method employs ion-pair extraction with di(2-ethylhexyl)phosphate followed by chromatography on a Zorbax SB C18 reversed-phase column. Fluorescence detection was used to identify the compounds of interest. The calibration curve was linear between 1 and 50 ng/mL albuterol hemisulfate salt (0.83 and 41.50 ng/mL albuterol base), and the limit of detection for a 1 mL sample was 1 ng/mL albuterol hemisulfate salt (0.83 ng/mL albuterol base). Serum levels of albuterol were quantified from guinea pigs that had received the drug by continuous subcutaneous infusion at a dose of 0.2 mg/kg/day for 1, 5 or 10 days, or 10 days followed by a 24 h washout period.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
ALP18910684	Albuterol Related Compound A		18910-68-4 (free base)	Price
AP51022709-A	Salbutamol hemisulfate salt		51022-70-9	Price

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