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Measurement of Protein Using Bicinchoninic Acid

Measurement of Protein Using Bicinchoninic Acid

P K Smith, R I Krohn, G T Hermanson, A K Mallia, F H Gartner, M D Provenzano, E K Fujimoto, N M Goeke, B J Olson, D C Klenk

Anal Biochem. 1985 Oct;150(1):76-85.

PMID: 3843705

Abstract:

Bicinchoninic acid, sodium salt, is a stable, water-soluble compound capable of forming an intense purple complex with cuprous ion (Cu1+) in an alkaline environment. This reagent forms the basis of an analytical method capable of monitoring cuprous ion produced in the reaction of protein with alkaline Cu2+ (biuret reaction). The color produced from this reaction is stable and increases in a proportional fashion over a broad range of increasing protein concentrations. When compared to the method of Lowry et al., the results reported here demonstrate a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts. The stability of the reagent and resulting chromophore also allows for a simplified, one-step analysis and an enhanced flexibility in protocol selection. This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP93523707	2-Keto-4-methylpentanoic-1-13C acid sodium salt		93523-70-7	Price

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