Home
Resources
Publications
Measurement of S-phase Fractions in Lymphoid Tissue Comparing Fresh Versus Paraffin-Embedded Tissue and 4',6'-diamidino-2 Phenylindole Dihydrochloride Versus Propidium Iodide Staining

Measurement of S-phase Fractions in Lymphoid Tissue Comparing Fresh Versus Paraffin-Embedded Tissue and 4',6'-diamidino-2 Phenylindole Dihydrochloride Versus Propidium Iodide Staining

R S Camplejohn, J C Macartney, R W Morris

Cytometry. 1989 Jul;10(4):410-6.

PMID: 2766887

Abstract:

S-phase fractions for 62 lymphoid biopsies were calculated, by means of flow cytometry, from both fresh and paraffin-embedded tissue. The purposes of this study were to determine whether significant differences were seen between S-phase estimates obtained from fresh and fixed tissue and to compare results obtained with two DNA dyes, namely 4'-6'-diamidino-2 phenylindole dihydrochloride (DAPI) and propidium iodide (PI). The 62 cases consisted of 38 cases of non-Hodgkin's lymphoma (NHL), 19 reactive samples, and 5 cases of Hodgkin's disease. Fifty-four of the samples showed DNA diploid profiles. A good agreement between S-phase results from fresh and fixed tissue was seen, with technical factors accounting for around 20% of the total variance. Using a paired t test, no significant difference was seen between fresh and fixed tissue for diploid cases, but there was a trend for S-phase estimates from fixed tissue to be higher. If all cases (including the eight DNA aneuploid samples) were included in the analysis this difference just reached statistical significance (P less than .05). In a subgroup of 19 of the cases, a comparison was performed on both fresh and fixed tissue of staining with DAPI and PI. A good agreement between results with both DNA stains was found on fresh and fixed tissue, with no significant differences being apparent, and stain-related factors accounted for only 10% of the total variance.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP28718903-A	4′,6-Diamidino-2-phenylindole dihydrochloride		28718-90-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: