Home
Resources
Publications
Measurement of Urinary 2,3-dinor-thromboxane B2 and Thromboxane B2 Using Bonded-Phase Phenylboronic Acid Columns and Capillary Gas Chromatography--Negative-Ion Chemical Ionization Mass Spectrometry

Measurement of Urinary 2,3-dinor-thromboxane B2 and Thromboxane B2 Using Bonded-Phase Phenylboronic Acid Columns and Capillary Gas Chromatography--Negative-Ion Chemical Ionization Mass Spectrometry

J A Lawson, A R Brash, J Doran, G A FitzGerald

Anal Biochem. 1985 Nov 1;150(2):463-70.

PMID: 4091270

Abstract:

The use of bonded-phase phenylboronic acid columns to selectively extract 2,3-dinor-thromboxane B2 and thromboxane B2 from urine is reported. The compounds were first derivatized as the methoxime and then applied to the phenylboronic acid columns. Subsequent purification by thin-layer chromatography and derivatization to the pentafluorobenzyl ester, trimethylsilyl ether followed by capillary gas chromatography-negative-ion chemical ionization mass spectrometry, monitoring specific ions, allows quantitation in the low-picogram/milliliter range. In healthy male volunteers, the median excretions of 2,3-dinor-thromboxane B2 and thromboxane B2 were 10.3 ng/h (range, 4.5-24 ng/h) and 2.8 ng/h (range, 0.5-7.3 ng/h), respectively. The method offers a noninvasive, specific approach to the study of thromboxane synthesis and platelet function in man. It is much less labor intensive than currently available methods employing electron-impact chromatography-mass spectrometry.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP177171163	3-(Trimethylsilyl)phenylboronic acid		177171-16-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: