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Measuring Lysosomal pH by Fluorescence Microscopy

Johnathan Canton, Sergio Grinstein

Methods Cell Biol. 2015;126:85-99.

PMID: 25665442

Abstract:

The technique of dual-wavelength ratio fluorescence microscopy provides a powerful tool to measure organellar pH. Unlike single-wavelength measurements, this method is unaffected by changes in focal plane, dye volume, and fluorophore bleaching, providing a quantitative and dynamic readout of the pH of subcellular compartments. This chapter describes the application of dual-wavelength ratio fluorescence microscopy to the measurement of lysosomal pH, highlighting its advantages and limitations. Probe selection, calibration methods, and salient aspects of the required hardware are discussed in detail.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP9004540-M Dextran 70 Calibration Dextran 70 Calibration 9004-54-0 Price
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