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Metabolic Labeling of Peptidoglycan With NIR-II Dye Enables In Vivo Imaging of Gut Microbiota

Metabolic Labeling of Peptidoglycan With NIR-II Dye Enables In Vivo Imaging of Gut Microbiota

Wei Wang, Qinglai Yang, Yahui Du, Xiaobo Zhou, Xiaochen Du, Qiuyue Wu, Liyuan Lin, Yanling Song, Fuyou Li, Chaoyong Yang, Weihong Tan

Angew Chem Int Ed Engl. 2020 Feb 10;59(7):2628-2633.

PMID: 31793153

Abstract:

Deepening our understanding of mammalian gut microbiota has been greatly hampered by the lack of a facile, real-time, and in vivo bacterial imaging method. To address this unmet need in microbial visualization, we herein report the development of a second near-infrared (NIR-II)-based method for in vivo imaging of gut bacteria. Using d-propargylglycine in gavage and then click reaction with an azide-containing NIR-II dye, gut microbiota of a donor mouse was strongly labeled with NIR-II fluorescence on their peptidoglycan. The bacteria could be readily visualized in recipient mouse gut with high spatial resolution and deep tissue penetration under NIR irradiation. The NIR-II-based metabolic labeling strategy reported herein, provides, to the best of our knowledge, the first protocol for facile in vivo visualization of gut microbiota within deep tissues, and offers an instrumental tool for deciphering the complex biology of these gut "dark matters".

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS77563	NIR-II dye			Price

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