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Metabolism of 2- And 3-tert-butyl-4-hydroxyanisole in the Rat (III): Metabolites in the Urine and Feces

Metabolism of 2- And 3-tert-butyl-4-hydroxyanisole in the Rat (III): Metabolites in the Urine and Feces

M Hirose, A Hagiwara, K Inoue, N Ito, H Kaneko, K Saito, H Matsunaga, N Isobe, A Yoshitake, J Miyamoto

Toxicology. 1988 Dec 16;53(1):33-43.

PMID: 3201475

Abstract:

The urinary and fecal metabolites of orally administered 2-tert-butyl-4-hydroxyanisole (2-BHA) and 3-tert-butyl-4-hydroxyanisole (3-BHA) in rats were identified. Samples of 2-day pooled urine and feces of rats given a single intragastric dose of 1 g/kg body wt of tert[butyl-14C]3-BHA (*Bu-3-BHA). tert[butyl-14C]2-BHA (*Bu-2-BHA), [methyl-14C]3-BHA (*Me-3-BHA) or [methyl-14C]-2-BHA (*Me-2-BHA) were analyzed by comparing thin-layer chromatography (TLC) retentions with authentic standards. Conjugated metabolites were identified after enzymatic hydrolysis. Proton magnetic resonance spectroscopy and electron impact mass spectrometry were used for confirmation of the authentic standards. In rats given 3-BHA, a major metabolite in the urine was 3-BHA-glucuronide with a smaller amount of tert-butylhydroquinone (TBHQ)-sulfate, while unchanged 3-BHA and 3-BHA-glucuronide were detected in the feces. In rats given 2-BHA, the main metabolites were the sulfate conjugates of 2-BHA, 4-tert-butyl-5-methoxy-1,2-benzoquinone (2-TBOQ) and the glucuronide of 2-BHA in the urine, while unchanged 2-BHA was found in the feces.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP121006-A	3-tert-Butyl-4-hydroxyanisole		121-00-6	Price
AP88324	2-tert-Butyl-4-hydroxyanisole		88-32-4	Price

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