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Metabolism of 4,7,10,13,16-docosapentaenoic Acid by Human Platelet Cyclooxygenase and Lipoxygenase

Metabolism of 4,7,10,13,16-docosapentaenoic Acid by Human Platelet Cyclooxygenase and Lipoxygenase

M M Milks, H Sprecher

Biochim Biophys Acta. 1985 Jun 14;835(1):29-35.

PMID: 3924108

Abstract:

Washed human platelets are shown to metabolize 4,7,10,13,16-docosapentaenoic acid into three major metabolites which were purified by reverse-phase HPLC. The mass spectra of the methyl ester-trimethylsilyl ether and ethyl ester-trimethylsilyl ether of compound A established it as delta 4-dihomo-thromboxane B2. Compound B was shown to be 14-hydroxy-4,7,10,12-nonadecatetraenoic acid, which is analogous to 12-hydroxy-5,8,10-heptadecatrienoic acid from arachidonic acid. Compound C was produced via an indomethacin-insensitive pathway and was identified as 14-hydroxy-4,7,10,12,16-docosapentaenoic acid. Time- and substrate-dependent studies showed that compounds A,B and C were produced approximately 10,15 and 65% of the extent to which thromboxane B2, 12-hydroxy-5,8,10-heptadecatrienoic acid and 12-hydroxy-5,8,10,14-eicosatetraenoic acid were produced, respectively, from arachidonic acid.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25182745	all-cis-4,7,10,13,16-Docosapentaenoic acid		25182-74-5	Price

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