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Metabolism of the Herbicide Chlortoluron by Human Cytochrome P450 3A4

Metabolism of the Herbicide Chlortoluron by Human Cytochrome P450 3A4

Z Mehmood, D E Kelly, S L Kelly

Chemosphere. 1995 Dec;31(11-12):4515-29.

PMID: 8574549

Abstract:

Studies were carried out to investigate the metabolism of herbicide chlortoluron in the microsomal fractions and whole cells of Saccharomyces cerevisiae expressing human cytochrome P450 3A4. Both whole cells and microsomal fractions of yeast expressing human cytochrome P450 3A4 exhibited a typical dithionite-reduced, CO-difference absorbance spectrum with maximum absorbance at 448 nm. Chlortoluron produced a type I binding spectrum with cytochrome P450 3A4 with a Ks value of 200 microM. Chlortoluron was metabolised into four metabolites; hydroxylated-N-monodemethylated, hydroxylated ring methylated, N-didemethylated and N-monodemethylated products. Chlortoluron metabolism was absolutely dependent on NADPH and no metabolism was observed in control transformants.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
CS35395	Chlortoluron-(N,N-dimethyl-d6)			Price

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