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Metabolomic Characterization of Rhubarb Species by Capillary Electrophoresis and Ultra-High-Pressure Liquid Chromatography

Metabolomic Characterization of Rhubarb Species by Capillary Electrophoresis and Ultra-High-Pressure Liquid Chromatography

Yufeng Jane Tseng, Chun-Ting Kuo, San-Yuan Wang, Hsiao-Wei Liao, Guan-Yuan Chen, Yuan-Ling Ku, Wei-Cheng Shao, Ching-Hua Kuo

Electrophoresis. 2013 Oct;34(19):2918-27.

PMID: 23580246

Abstract:

This study developed CE and ultra-high-pressure LC (UHPLC) methods coupled with UV detectors to characterize the metabolomic profiles of different rhubarb species. The optimal CE conditions used a BGE with 15 mM sodium tetraborate, 15 mM sodium dihydrogen phosphate monohydrate, 30 mM sodium deoxycholate, and 30% ACN v/v at pH 8.3. The optimal UHPLC conditions used a mobile phase composed of 0.05% phosphate buffer and ACN with gradient elution. The gradient profile increased linearly from 10 to 21% ACN within the first 25 min, then increased to 33% ACN for the next 10 min. It took another 5 min to reach the 65% ACN, then for the next 5 min, it stayed unchanged. Sixteen samples of Rheum officinale and Rheum tanguticum collected from various locations were analyzed by CE and UHPLC methods. The metabolite profiles of CE were aligned and baseline corrected before chemometric analysis. Metabolomic signatures of rhubarb species from CE and UHPLC were clustered using principle component analysis and distance-based redundancy analysis; the clusters were not only able to discriminate different species but also different cultivation regions. Similarity measurements were performed by calculating the correlation coefficient of each sample with the authentic samples. Hybrid rhizome was clearly identified through similarity measurement of UHPLC metabolite profile and later confirmed by gene sequencing. The present study demonstrated that CE and UHPLC are efficient and effective tools to identify and authenticate herbs even coupled with simple detectors.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP145224926	Sodium deoxycholate monohydrate		145224-92-6	Price

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