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Metabolomic Profiles of Bovine Mammary Epithelial Cells Stimulated by Lipopolysaccharide

Metabolomic Profiles of Bovine Mammary Epithelial Cells Stimulated by Lipopolysaccharide

Yixin Huang, Liuhong Shen, Jing Jiang, Qipin Xu, Zhengzhong Luo, Qiao Luo, Shumin Yu, Xueping Yao, Zhihua Ren, Yanchun Hu, Yongxin Yang, Suizhong Cao

Sci Rep. 2019 Dec 13;9(1):19131.

PMID: 31836784

Abstract:

Bovine mammary epithelial cells (bMECs) are the main cells of the dairy cow mammary gland. In addition to their role in milk production, they are effector cells of mammary immunity. However, there is little information about changes in metabolites of bMECs when stimulated by lipopolysaccharide (LPS). This study describes a metabolomics analysis of the LPS-stimulated bMECs to provide a basis for the identification of potential diagnostic screening biomarkers and possible treatments for bovine mammary gland inflammation. In the present study, bMECs were challenged with 500 ng/mL LPS and samples were taken at 0 h, 12 h and 24 h post stimulation. Metabolic changes were investigated using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF MS) with univariate and multivariate statistical analyses. Clustering and metabolic pathway changes were established by MetaboAnalyst. Sixty-three differential metabolites were identified, including glycerophosphocholine, glycerol-3-phosphate, L-carnitine, L-aspartate, glutathione, prostaglandin G2, α-linolenic acid and linoleic acid. They were mainly involved in eight pathways, including D-glutamine and D-glutamic acid metabolism; linoleic acid metabolism; α-linolenic metabolism; and phospholipid metabolism. The results suggest that bMECs are able to regulate pro-inflammatory, anti-inflammatory, antioxidation and energy-producing related metabolites through lipid, antioxidation and energy metabolism in response to inflammatory stimuli.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP51982366	Prostaglandin G2		51982-36-6	Price

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