Home
Resources
Publications
Methods for Synchronizing Cells at Specific Stages of the Cell Cycle

Methods for Synchronizing Cells at Specific Stages of the Cell Cycle

J Jackman, P M O'Connor

Curr Protoc Cell Biol. 2001 May;Chapter 8:Unit 8.3.

PMID: 18228388

Abstract:

Exponentially growing cells are asynchronous with respect to the cell cycle stage. Detection of cell cycle-related events is improved by enriching the culture for cells at the stage during which the particular event occurs. Methods for synchronizing cells are provided here, including those based on morphological features of the cell (mitotic shake-off), cellular metabolism (thymidine inhibition, isoleucine depravation), and chemical inhibitors of cell progression in G1 (lovastatin), S (aphidicolin, mimosine), and G2/M (nocodazole). Applications of these methods and the advantages and disadvantages of each are described.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP38966211-A	Aphidicolin		38966-21-1	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: